“A draft Musa balbisiana genome sequence for molecular genetics in polyploid, inter- and intra-specific Musa hybrids”

Table 1 overview of mean mapped read depth per chromosome, and the derived B-genome chromosome lengths following alignment to the A-genome

Sequence	‘Pahang’ Reference length/bp	Mapped reads count	Mean mapped read depth	‘PKW’ mapped read length/bp	% of A -genome coverage
chr1	27,573,629	11,164,973	36.9	22,038,404	79.9
chr2	22,054,697	8,996,066	37.1	17,349,238	78.7
chr3	30,470,407	12,275,431	36.6	24,161,952	79.3
chr4	30,051,516	13,632,301	41.3	24,656,528	82.0
chr5	29,377,369	12,398,532	38.2	23,648,591	80.5
chr6	34,899,179	16,499,244	43.0	27,831,592	79.7
chr7	28,617,404	13,123,105	42.1	22,212,853	77.6
chr8	35,439,739	14,663,790	37.7	27,665,716	78.1
chr9	34,148,863	14,480,461	38.5	25,900,723	75.8
chr10	33,665,772	14,673,154	39.6	25,230,959	74.9
chr11	25,514,024	11,897,139	42.3	20,721,546	81.2
chrUn_random	141,147,818	100,613,680	64.2	80,013,141*	56.7*
total counts / averages	472,960,417	244,417,876	41.4	341,431,243*	78.9**

*The assembled PKW consensus sequence for ‘B-chrUn_random’ derived from read mapping is 80.01 Mb, but for molecular genetic studies, all gaps were filled with ‘N’s to prevent artificial concatenation and ‘bridging’ of mapped reads across unrelated genic regions. Therefore the working sequence length is 141.13 Mbp, essentially the same as for the homoeologous A-genome sequence, and the working PKW consensus genome length is 402.547 Mbp.
**The mean% of A-genome chromosome length is calculated only for chromosomes 1 – 11.

ISSN: 1471-2164