Figure 3

Methylation and expression analyses of DNMT1 in human placenta . (A) CGI 89 is located at the promoter of DNMT1. (B) Bisulfite treatment and cloning confirmed monoallelic methylation. (C) Sequencing of clones from the heterozygous individual fN599 showed that the maternal allele (T allele) was associated with methylated CpGs, while the paternal allele (G allele) was associated with unmethylated CpGs. (D) DNMT1 was paternally expressed in humans. Arrow depicts the SNP location for each sample. In all four samples, the fetal DNA was heterozygous and the maternal DNA was homozygous. Fetal RNA showed monoallelic expression from the paternal allele.