Figure 2

Estimates of RNA yields in the smallest diagnostic biopsies. (a) RNA from 46 formalin-fixed prostate needle core biopsy specimens collected between 2003 and 2008 by the Cleveland Clinic was extracted and RNA yields were measured using the RiboGreen method. Tumor volumes were measured and all results were adjusted to represent RNA yields in the target tumor volume of 0.0225 mm³ (1 mm tumor length x 0.75 mm width of diagnostic biopsies x 0.030 mm tumor depth). (b) AUA low and intermediate risk patients treated with radical prostatectomy at Cleveland Clinic between 1999 and 2007 with available biopsy tissues were included in the study. In total, RNA from 167 diagnostic biopsies was extracted and RNA yields were measured using the RiboGreen method. Tumor volumes were measured and all results were adjusted to represent RNA yields in the target tumor volume of 0.0225 mm³ (1 mm tumor length x 0.75 mm width of diagnostic biopsies x 0.030 mm tumor depth).