Skip to main content

Table 2 Limits of quantitation and detection

From: Analytical validation of the Oncotype DX prostate cancer assay – a clinical RT-PCR assay optimized for prostate needle biopsies

Gene Assay

Est. LOQ (Cp)

Est. LOD (Cp)

ARF1

33.4

38.9

ATP5E

33.6

40.0

AZGP1

34.7

40.0

BGN

35.3

36.3

CLTC

34.3

40.0

COL1A1

33.2

37.0

FAM13C1

35.3

40.0

FLNC

32.7

40.0

GPS1

33.5

40.0

GSN

33.4

40.0

GSTM2

33.2

40.0

KLK2

29.4

40.0

PGK1

35.4

40.0

SFRP4

34.8

40.0

SRD5A2

33.5

36.6

TPM2

33.4

40.0

TPX2

34.4

36.3

  1. Analytical sensitivity (estimated Limits of Quantitation and Limits of Detection) for each of the 17 genes comprising the GPS derived from the 15-point dilution series.