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Figure 5 | BMC Genomics

Figure 5

From: Host-Mycobacterium avium subsp. paratuberculosis interactome reveals a novel iron assimilation mechanism linked to nitric oxide stress during early infection

Figure 5

MAP utilized the SugA-SugB-SugC pathway for trehalose recycling in MAC-T cells. Differentially expressed MAP transcripts (P < 0.5) were categorized into networks using STRING ver. 9. Networks were cross-referenced against the Tuberculist, KEGG, and mycobacteria literature. Network analyses identified the upregulation of the SugA-SugB-SugC pathway in MAP inside MAC-T cells. Organization of the sugABC and uspABC operons in MAP and M. tuberculosis H37Rv (above). MAP UspA is a periplasmic sugar binding protein that forms a lipid anchor and binds to trehalose. Trehalose is transported into the MAP cell by a transmembrane protein (heterodimer of SugA and SugB) and SugC, a terminal ATP-binding cytoplasmic protein. It is hypothesized that trehalose interacts with mycolic acids by a currently unknown mechanism to produce TMM that is exported into the extracellular milieu to re-start the recycling process. Abbreviations: AG = arabinogalactan, PG = peptidoglycan, CM = cytoplasmic membrane, TMM = trehalose-containing molecules and TDM = trehalose dimycolate. Modified figure is based on the pathway/figure described by Kalscheuer et al.[58].

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