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Figure 1 | BMC Genomics

Figure 1

From: Histoplasma yeast and mycelial transcriptomes reveal pathogenic-phase and lineage-specific gene expression profiles

Figure 1

Transcriptional evidence-based pipeline for assembly of Histoplasma G186A gene models. RNA-seq data was used to generate spliced alignment models to the reference genome (gene set 1 and 2) as well as de novo assembly into contiguous transcripts (gene set 3′). Gene sets 1 and 2 were updated by PASA (red triangles) using the de novo transcript assembly. The three gene sets were integrated and subjected to an additional update using the de novo mRNA assembly. The resultant gene set was manually refined after inspection of the models for genes with unusual characteristics (introns greater than 350 bp or intergenic distances less than 500 bp). The final gene models were subsequently categorized as single copy genes, repetitive genes, or genes with low experimental support (gene models based on low numbers of RNA-seq reads).

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