Annotation of TSS using small RNA sequencing. Frequency distribution of RNA fragment 5′ (red) and 3′ (blue) termini aligning sense to mRNA strand. We observed protection of the initiation (green box) with 3 nt periodicity to the stop codon (red) box. Distribution of RNA fragments 5′ (red) and 3′ (blue) to predict transcription start sites (TSS) for genome annotation. Top panel indicates the protection of the Shine-Dalgarno (SD) sequence.