Figure 2

Analysis of the diversity of sequences suggests that a higher depth of sequencing is required for quantitative results for sRNA libraries. A.Rarefaction curve represents the number of different reads found at the indicated number of reads. The Y axis represents the number of different sequences that could be determined from each sample and the X axis represent the number of random sequences from our sRNA libraries. B. miR393 is regulated by nitrate in qRT-PCR experiments. We show results for three biological replicates. We show standard errors for each bar. The asterisk indicates means that differ significantly (p < 0.05). C. Rarefaction curve of unique reads that match annotated genes from poly-A + libraries.