Table 2 Illumina sequencing identifies novel miRNAs
miRNA | Chr | miRNA Start | miRNA End | Mature Sequence | miRNA* | miRNA previoulsy reported | miRNA located in |
|---|---|---|---|---|---|---|---|
miR5640 | 1(−) | 1653540 | 1653560 | AUGAGAGAAGGAAUUAGAUUC | YES | ath-miR5640 [47] | AT1G05570.1 intron |
ath-MIR472-5p | 1(−) | 4182266 | 4182286 | AUGGUCGAAGUAGGCAAAAUC | NO | Novel | ath-MIR472 stem loop |
ath-MIR8166 | 1(−) | 4525316 | 4525337 | AGAGAGUGUAGAAAGUUUCUCA | NO | Novel | Intergenic region AT1G13240-AT1G13245 |
miR5654-3p | 1(+) | 11786350 | 11786371 | GAAGAUGCUUUGGGAUUUAUUU | NO | AT1G32583.1, 5'UTR | |
ath-MIR829-5p | 1(−) | 11834153 | 11834173 | ACUUUGAAGCUUUGAUUUGAA | YES | Novel | ath-MIR829 stem loop |
miR5014a | 1(+) | 24554009 | 24554029 | UGUUGUACAAAUUUAAGUGUA | YES | AT1G65960.1 intron | |
ath-MIR840-3p | 1(−) | 771385 | 771405 | UUGUUUAGGUCCCUUAGUUUC | YES | Novel | ath-MIR840 stem loop |
ath-MIR398a-3p | 2(+) | 1040948 | 1040968 | AAGGAGUGGCAUGUGAACACA | YES | Novel | ath-MIR398a stem loop |
ath-MIR8180 | 2(+) | 2063980 | 2063998 | UGCGGUGCGGGAGAAGUGC | NO | Novel | Intergenic region AT2G05580-AT2G05590 |
ath-MIR8175 | 2(+) | 3740938 | 3740957 | GAUCCCCGGCAACGGCGCCA | NO | Novel | Intergenic region AT2G09880-AT2G09890 |
ath-MIR396a-3p | 2(−) | 4142331 | 4142351 | GUUCAAUAAAGCUGUGGGAAG | YES | Novel | ath-MIR396a stem loop |
ath-MIR8168 | 2(+) | 5080690 | 5080710 | AGGUGCUGAGUGUGCUAGUGC | NO | Novel | Intergenic region AT2G12490-AT2G12500 |
ath-MIR5632-5p | 2(−) | 8392588 | 8392608 | UUGAUUCUCUUAUCCAACUGU | YES | Novel | ath-MIR5632 stem loop |
ath-MIR8167a | 2(+) | 8894985 | 8895006 | AGAUGUGGAGAUCGUGGGGAUG | NO | Novel | Intergenic region AT2G20620-AT2G20625 |
miR5995b | 2(−) | 10026977 | 10026997 | AAAGAUGCAGAUCAUAUGUCC | YES | ath-miR5995b [63]) | Intergenic region AT2G23540-AT2G23550 |
ath-MIR831-5p | 2(+) | 10247259 | 10247280 | AGAAGCGUACAAGGAGAUGAGG | NO | Novel | ath-MIR831 stem loop |
miR5637 | 2(−) | 12270195 | 12270216 | UAGAGGAAAAUAUAGAGUUGGG | NO | ath-miR5637 [47] | Intergenic region AT2G28620-AT2G28625 |
ath-MIR8170.1 | 2(+) | 14100020 | 14100040 | AUAGCAAAUCGAUAAGCAAUG | YES | Novel | AT2G33255.1 intron |
ath-MIR8170.2 | 2(+) | 14100079 | 14100099 | UUGCUUAAAGAUUUUCUAUGU | YES | Novel | AT2G33255.1 intron |
ath-MIR160a-3p | 2(+) | 16340342 | 16340362 | GCGUAUGAGGAGCCAUGCAUA | YES | Novel | ath-MIR160a stem loop |
ath-MIR8171 | 2(+) | 16890466 | 16890486 | AUAGGUGGGCCAGUGGUAGGA | NO | Novel | AT2G40440.1 intron |
ath-MIR166a-5p | 2(+) | 19176128 | 19176148 | GGACUGUUGUCUGGCUCGAGG | YES | Novel | ath-MIR166 stem loop |
ath-MIR408-5p | 2(+) | 19319866 | 19319886 | ACAGGGAACAAGCAGAGCAUG | YES | Novel | ath-MIR408 stem loop |
miR5650 | 2(+) | 19686959 | 19686979 | UUGUUUUGGAUCUUAGAUACA | YES | ath-miR5650 [47] | AT2G48140.1 intron |
miR173-5p | 3(+) | 8236161 | 8236182 | UUCGCUUGCAGAGAGAAAUCAC | YES | ath-miR173-5p stem loop | |
ath-MIR8169 | 3(+) | 8836359 | 8836379 | AUAGACAGAGUCACUCACAGA | NO | Novel | Intergenic region AT3G24340-AT3G24350 |
ath-MIR8183 | 3(−) | 11747799 | 11747819 | UUUAGUUGACGGAAUUGUGGC | NO | Novel | AT3G30110.1, pseudogene |
ath-MIR8165 | 3(−) | 16538510 | 16538530 | AAUGGAGGCAAGUGUGAAGGA | NO | Novel | Intergenic region AT3G45170-AT3G45180 |
ath-MIR8174 | 3(−) | 16589431 | 16589451 | AUGUGUAUAGGGAAGCUAAUC | NO | Novel | Intergenic region AT5G38460-AT5G38470 |
miR5651 | 3(+) | 17178489 | 17178509 | UUGUGCGGUUCAAAUAGUAAC | YES | ath-miR5651 [47] | Intergenic region AT3G46616-AT3G46620 |
ath-MIR8167b | 3(−) | 8894985 | 8895006 | AGAUGUGGAGAUCGUGGGGAUG | NO | Novel | Intergenic region AT3G47410-AT3G47420 |
ath-MIR8167c | 3(−) | 17469946 | 17469967 | AGAUGUGGAGAUCGUGGGGAUG | NO | Novel | Intergenic region AT3G50700-AT3G50710 |
miR5633 | 3(+) | 19544786 | 19544807 | AUGAUCAUCAGAAAACAGUGAU | NO | ath-miR5633 [47]) | Intergenic region AT3G52730-AT3G52740 |
ath-MIR393b-3p | 3(+) | 20691778 | 20691798 | AUCAUGCGAUCUCUUUGGAUU | YES | Novel | ath-MIR393 stem loop |
ath-MIR8182 | 3(+) | 22678166 | 22678187 | UUGUGUUGCGUUUCUGUUGAUU | NO | Novel | AT3G61270.1, 5'UTR |
ath-MIR166b-5p | 3(+) | 22922212 | 22922232 | GGACUGUUGUCUGGCUCGAGG | YES | Novel | ath-MIR166 stem loop |
ath-MIR8172 | 4(−) | 7102572 | 7102592 | AUGGAUCAUCUAGAUGGAGAU | YES | Novel | Intergenic region AT4G11800-AT4G11810 |
ath-MIR8179 | 4(−) | 7161930 | 7161950 | UGACUGCAUUAACUUGAUCGU | NO | Novel | AT4G1192.1 intron |
ath-MIR8176 | 4(+) | 11795199 | 11795219 | GGCCGGUGGUCGCGAGAGGGA | NO | Novel | Intergenic region AT4G22320-AT4G22330 |
ath-MIR8178 | 4(+) | 18087285 | 18087305 | UAACAGAGUAAUUGUACAGUG | NO | Novel | AT4G38760.1 intron |
ath-MIR8184 | 5(−) | 3311974 | 3311994 | UUUGGUCUGAUUACGAAUGUA | NO | Novel | Intergenic region AT5G10504-AT5G10510 |
miR5629 | 5(+) | 3802933 | 3802954 | UUAGGGUAGUUAACGGAAGUUA | NO | ath-miR5629 [47] | Intergenic region AT5G11790-AT5G11800 |
ath-MIR865.2 | 5(+) | 5169992 | 5170011 | UCUGGGAUGAAUUUGGAUCU | NO | Novel | ath-MIR865 stem loop |
miR1888 | 5(+) | 7168879 | 7168899 | UAAGUUAAGAUUUGUGAAGAA | NO | AT5G21100.1 intron | |
ath-MIR8173 | 5(−) | 7478572 | 7478592 | AUGUGCUGAUUCGAGGUGGGA | NO | Novel | Intergenic region AT5G22510-AT5G22520 |
ath-MIR8177 | 5(−) | 9362634 | 9362655 | GUGUGAUGAUGUGUCAUUUAUA | NO | Novel | Intergenic region AT5G26617-AT5G26620 |
miR5638b | 5(+) | 14100017 | 14100037 | ACAGUGGUCAUCUGGUGGGCU | NO | ath-miR5638b [47] | Intergenic region AT5G35945-AT5G35950 |
ath-MIR160c-3p | 5(−) | 19009095 | 19009115 | CGUACAAGGAGUCAAGCAUGA | YES | Novel | ath-MIR160c stem loop |
ath-MIR870-5p.1 | 5(−) | 21395592 | 21395612 | UUAGAAUGUGAUGCAAAACUU | NO | Novel | ath-MIR870 stem loop |
ath-MIR870-5p.2 | 5(−) | 21395604 | 21395624 | AAGAACAUCAAAUUAGAAUGU | NO | Novel | ath-MIR870 stem loop |
ath-MIR8181 | 5(−) | 21641289 | 21641308 | UGGGGGUGGGGGGGUGACAG | NO | Novel | AT5G5333.1, intron |