Figure 3
New superfamilies of conopeptides. Top panel shows an identity cladogram in which signal sequences of conopeptides matching only pro- and mature regions (in grey) have been aligned with consensus signal regions of known superfamilies (in red). Percentage value between brackets following the superfamily name measures the intraspecific conservation of members populating this family (the “*” symbol means that only one sequence is part of the family). Bottom panel shows the 33 new precursor sequences spread over the 13 new superfamilies. Red rectangles enclose the signal sequences determined with SignalP 4.0. The number following the precursor name is the precursor sequence frequency among the input data set. Bold partial sequences represent the peptide fragments retrieved by MS/MS sequencing of milked venom.