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Figure 1 | BMC Genomics

Figure 1

From: Pseudo-Sanger sequencing: massively parallel production of long and near error-free reads using NGS technology

Figure 1

Construction of pseudo-Sanger sequences. Genomic DNA (a) is randomly sheared into fragments (b) of a wide range of sizes. Libraries are constructed for each band with a step size of ~100 bp and subjected to Illumina paired-end sequencing (c). Anchor reads (ARs) come from the library with the largest fragments. The rest are supporting reads (SRs). Each AR is aligned with all SRs that are likely to fill in its internal gap (d). Local assembly is then performed on of the AR plus the associated SRs to construct a pseudo-Sanger sequence. The resulting pseudo-Sanger sequences cover the entire genome much like other WGS reads (e).

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