Figure 2

Bar charts of normalised peak areas of six intermediates of arginine biosynthesis after HPLC-ESI-qTOF analysis. C. glutamicum ATCC 13032 (WT) and seven double deletion mutants were cultivated with l-arginine until exponential phase. Then l-arginine was removed and cells were further incubated to accumulate intracellular metabolites. The boxes in each diagram indicate the respective intracellular compound and its mass-to-charge ratio. Peak detection and integration was performed on base peak chromatograms (BPC) of m/z-values of [M + H]⁺ ions. Values that are significantly different from the wildtype level (Student’s T-test p < 0.05) are indicated by an asterisk. Error bars represent standard deviations of four biological replicates.