Table 2 Bacterial strains and plasmids

Strains

E. coli JM109

F´ traD36, proA⁺B⁺, lacI^q Δ(lacZ,)M15/ Δ(lac-proAB), glnV44,e14^-, gyrA96, recA1, relA1, endA1, thi, hsdR17

New EnglandBiolabs

E. coli JM109 ∆argA

JM109 with deleted argA gene

This study

C. glutamicum ATCC 13032

Wild type (WT), Nx^r

American type culture collection

∆argRC

C. glutamicum ATCC 13032 with deleted argR and argC genes

This study

∆argRJ

C. glutamicum ATCC 13032 with deleted argR and argJ genes

This study

∆argRB

C. glutamicum ATCC 13032 with deleted argR and argB genes

This study

∆argRD

C. glutamicum ATCC 13032 with deleted argR and argD genes

This study

∆argFR

C. glutamicum ATCC 13032 with deleted argR and argF genes

[40]

∆argRG

C. glutamicum ATCC 13032 with deleted argR and argG genes

This study

∆argRH

C. glutamicum ATCC 13032 with deleted argR and argH genes

This study

∆cg3035

C. glutamicum ATCC 13032 with deleted cg3035 gene

This study

Plasmids

pK18mobsacB

sacB, lacZα, Km^r , mcs mobilizable vector, allows for selection of double crossover in C. glutamicum

[41]

pZ8-1

E. coli-C. glutamicum shuttle expression vector, P_tac, Km^r

[42]

pZ8-2

modified pZ8-1 where the multiple cloning site was exchanged by a sole Bam HI-site and a consensus RBS was included

This study

pZ8-1::argA

pZ8-1 containing the argA structural gene from E. coli

This study

pZ8-1::cg3035

pZ8-1 containing the cg3035 structural gene from C. glutamicum ATCC 13032

This study

pZ8-1::argJ

pZ8-1 containing the argJ structural gene from C. glutamicum ATCC 13032

This study