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Figure 3 | BMC Genomics

Figure 3

From: De novo transcriptome profiling uncovers a drastic downregulation of photosynthesis upon nitrogen deprivation in the nonmodel green alga Botryosphaerella sudeticus

Figure 3

Coherence of transcription levels in ribosomal protein-encoding ESTs in B. sudeticus . (A) Distribution of ESTs encoding eRPs and aRPs based on rank by level. The heat-map shows the EST occurrence that is binned by an average sliding window of 11 consecutive ranks and normalized to 1 for each pathway or component. The color key for percent levels is shown at the bottom. The box-plot indicates the minimum (min), first quartile (q1), median (mid), third quartile (q3), and maximum (max) of eRP and aRP EST levels by rank. (B) Occurrence densities of eRP and aRP ESTs. Upper table shows the range of ESTs based on rank by level. Four ranges are shown: first 50% (R-F50), middle 50% (R-M50), and second 50% (R-S50) of eRP- or aRP-ESTs and top 10% (R-Top) of most abundant ESTs based on rank by level. Mid table shows the occurrence density of eRP- or aRP-ESTs. Bottom table shows the relative occurrence density in 3 half-ranges containing 50% of the eRP or aRP-ESTs. Coherent transcription or relative density in one of the 3 half-ranges is 2-fold above background and p-value is less than 0.05. Different levels of statistical significance are indicated as triple asterisk (***), double asterisk (**), and single asterisk (*) for p-value < 0.001, < 0.01, and < 0.05, respectively. (C) Distribution of eRP- and aRP-ESTs based on rank by ratio. Top panel shows the heat-map and box-plot of eRP- and aRP-EST distribution based on rank by ratio. Bottom table shows the relative occurrence density of eRP- and aRP-ESTs in 3 half-ranges and in top or bottom of the most differentially transcribed ESTs. Levels of statistical significance are indicated as (B). (D) Distribution of eRP- and aRP-EST ratios. Change of median ratios (ΔM) of eRP- and aRP-ESTs compared to background is indicated.

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