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Table 1 Statistics of categorization and abundance of DGE tags

From: Use of digital gene expression to discriminate gene expression differences in early generations of resynthesized Brassica napus and its diploid progenitors

Summary

 

B. rapa

B. oleracea

B. napus- F1

B. napus- F2

B. napus- F3

B. napus- F4

B. napus- N

Raw Data

Total

6178564

6059222

6155227

6092805

6142098

5938583

5964594

Raw Data

Distinct Tag

293575

243895

331406

275597

284202

272986

269285

Clean tag

Total number

6018254

5930726

6022170

5950123

5991210

5798939

5823113

Clean tag

Distinct Tag number

133499

116771

199428

134403

134721

134857

128967

Tag Mapping to Gene

Total number

1964909

1747843

3025405

2354059

1652733

2295897

2253347

Tag Mapping to Gene

Distinct Tag number

44267

36220

88270

49781

42613

51122

45358

Unambiguous Tag Mapping to Gene

Total number

1679848

1475050

2495411

2016182

1415736

1971591

1924944

Unambiguous Tag Mapping to Gene

Total % of clean tag

27.91%

24.87%

41.44%

33.88%

23.63%

34.00%

33.06%

Unambiguous Tag Mapping to Gene

Distinct Tag number

39414

31933

79648

44456

37996

45762

40561

Unambiguous Tag Mapping to Gene

Distinct Tag % of clean tag

29.52%

27.35%

39.94%

33.08%

28.20%

33.93%

31.45%

Tag-mapped Genes

number

19023

18547

24383

20659

18881

20692

19955

Tag-mapped Genes

% of ref genes

46.20%

45.05%

59.22%

50.17%

45.86%

50.26%

48.47%

Unambiguous Tag-mapped Genes

number

16574

15970

22059

18155

16479

18196

17448

Unambiguous Tag-mapped Genes

% of ref genes

40.25%

38.79%

53.58%

44.09%

40.02%

44.19%

42.38%

Mapping to Genome

Total number

2437918

2105332

1776978

2290544

2658991

2232572

2164464

Mapping to Genome

Total % of clean tag

40.51%

35.50%

29.51%

38.50%

44.38%

38.50%

37.17%

Mapping to Genome

Distinct Tag number

44076

30703

49898

42334

45680

42590

40689

Mapping to Genome

Distinct Tag % of clean tag

33.02%

26.29%

25.02%

31.50%

33.91%

31.58%

31.55%

Unknown Tag

Total number

1615427

2077551

1219787

1305520

1679486

1270470

1405302

Unknown Tag

Total % of clean tag

26.84%

35.03%

20.25%

21.94%

28.03%

21.91%

24.13%

Unknown Tag

Distinct Tag number

45156

49848

61260

42288

46428

41145

42920

Unknown Tag

Distinct Tag % of clean tag

33.82%

42.69%

30.72%

31.46%

34.46%

30.51%

33.28%

  1. Clean tags are tags after filtering dirty tags from raw data. Distinct tags are different kinds of tags and unambiguous tags are the reminder clean tags after removing tags mapped to reference genome.