Figure 4
Decreased hepatogenesis characters in yHCCs but increased ESC traits in advanced HCCs. (A) Forfeiting of liver differentiation program in yHCCs. (Left) A PCA plot using genes differentiating the H9 ESCs and day 20 hepatic differentiated progenies (q < 10-4). The transcriptome drift directions during hepatic differentiation are indicated by an arrow. Hepatic_ESC: day 20 (d20) hepatic differentiated progenies. (right) Relationships between liver precursors and HCCs of different age groups. (B) Relationships between ESC and HCC of different histopathological stages. (Left) A PCA plot based on 977 probe sets genes distinguishing early (T1) and late (T3) HCCs from elderly patients. (Right) Relationships between ESC and T1/T3 HCCs. (C) Dedifferentiation-like transcriptome reprogramming during the progression of HCV-related HCC. (Left) A PCA plot using genes differentiating very early (ve) and advanced (a) HCC (q < 0.01, 1700 probe sets). The orange arrow represents the transcriptome drift direction during HCC progression. eHCC, early HCC; aHCC, advanced HCC; Hepatic_iPS cells: day 20 hepatic differentiated progenies of iPS cells. (Right) Relationships between ESC and HCC at different clinical stages.