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Table 2 Assembly results of 454 data by using Newbler default and optimized parameters

From: De novo transcriptome analysis using 454 pyrosequencing of the Himalayan Mayapple, Podophyllum hexandrum

Description

Newbler (Default parameter)

Newbler (Optimized parameter)

 

Contigs

Singlets

Contigs

Singlets

Number of contigs/Singlets

3372

62937

12940

27440

Total bases of contigs/Singlets(bp)

1143488

12156572

5928039

7033852

Mean contig/Singlet length

339.112

193.154

458.117

256.335

Contig/Singlet N50

339

190

463

240

Max Contig/Singlet size

2167

297

4915

399