Real-time PCR validation of microarray result. Selected miRNAs were measured by real-time PCR in tumor tissues and paired adjacent benign tissues (n = 20). Horizontal bars represent the mean. P vlaues were measured by paired t-test. (A) real-time PCR analysis for up-regulated miRNAs in microarray result. (B) real-time PCR analysis for down-regulated miRNAs in microarray result.