Figure 3

Functional annotation of genes regulated in WAT 24 hours after onset of fasting reveals activation of transcription regulatory components. (A) and (B) DAVID functional clustering was performed with genes upregulated (A) or downregulated (B) by fasting (1.3x, FDR5). Shown are the numbers of unique genes (inside of bars) and the enriched clusters (left of bars with contributing DAVID domains in parentheses). The x-axis shows the cluster enrichment scores representing a geometric mean (−log) of p-values of entities in each cluster. Clusters with an enrichment score larger than three are shown. CC = cellular components, MF = molecular functions, BP = biological processes. (C) GSEA enrichment plots are generated by ranking all genes in the dataset by expression values, mapping transcription factor (TF) sub-categories (bZIP = basic leucine zippers; Fox = forkhead box), cofactors, and chromatin remodelers, and calculating a cumulative enrichment score (green line). NES = normalized enrichment score, FDR = false discovery rate.