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Figure 6 | BMC Genomics

Figure 6

From: Differential expression of CPKs and cytosolic Ca2+ variation in resistant and susceptible apple cultivars (Malus x domestica) in response to the pathogen Erwinia amylovora and mechanical wounding

Figure 6

Intracellular Ca2+variations in GD and M7 leaf cells following pathogen infection and mechanical damage. Leaves were treated with calcium orange for 1 hr and then infected with E. amylovora. The cytosolic Ca2+ concentration of leaf cells was determined 1 hr, 2 hr and 6 hrs after infection. Mechanically damaged (MD) leaves served as a control for both genotypes. In the lower panel, the bar represents the relative percentage of calcium orange fluorescence in at least 3 replicates. Asterisk indicate significant (p <0.05) differences. Scale bars for Figures A-F and H-O = 100 μm, scale bars for Figures G and P = 400 μm.

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