Generation of RNA-sequencing libraries. (a) Developmental stages of Xenopus tropicalis. (b) RPKM distribution across six developmental time-points. Numbers on the x-axis are Xenopus tropicalis Nieuwkoop and Faber developmental stages, Oocyte (Oo), stage 6, stage 9, stage 12, stage 16 and stage 30. (c) Heat map to show Pearson correlation of expression (RPKM) between all 9 RNA-seq libraries. (d) Scatter plots to show stage specific Pearson correlation between RNA-seq data generated using two different methods. Log2 RPKM values are plotted on x and y axis respectively. PolyA+ (RNA harvested with double PolyA+ selection), RZ (ribosomal rRNA depleted-total RNA).