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Figure 4 | BMC Genomics

Figure 4

From: The periodontal pathogen Porphyromonas gingivalis changes the gene expression in vascular smooth muscle cells involving the TGFbeta/Notch signalling pathway and increased cell proliferation

Figure 4

The expression of selected genes and proteins in AoSMCs stimulated with P. gingivalis . Quantitative real-time PCR results demonstrate relative transcription for TGF-β1 (A), CTGF (B), SMAD3 (C), NOTCH1 (D), and HEY1 (E) of AoSMCs stimulated (P. g) or unstimulated (control) with P. gingivalis at 10 MOI for 24 h. All these results were normalized by the gene expression level of the house-keeping gene GAPDH. Representative western blot showing NOTCH1 protein expression levels of AoSMCs exposed to P. gingivalis at 8 and 10 MOI (F). Quantification of NOTCH1 protein expression levels by densitometry is shown in (G). NOTCH1 density signals were normalized to GAPDH signal values. P. gingivalis resulted in a dose-dependent (MOI 8 and 10) induction of TGF-β1 protein expression (H). *, p < 0.05; **, p < 0.005; ***, p < 0.0001. A, n = 10; B-E, n = 6; F, G, n = 3; H, n = 3-4.

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BMC Genomics

ISSN: 1471-2164

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