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Table 1 Comparison of pTAL4 and pTAL7 vector systems

From: A modified TALEN-based system for robust generation of knock-out human pluripotent stem cell lines and disease models

 

pTAL4

pTAL7

Site for GoldenGate TALEN Assembly

Esp3I

Esp3I

Bacterial Resistance

Ampicillin

Kanamycin

Screening for successful cloning

LacZ

LacZ

TALEN architecture

Full length Voytas et al. [13]

Truncated C- & N-termini similar to Miller et al. [6]

FokI nuclease

Wild-type

Wild-type

Expression system

Yeast

Mammalian cells (CAG promoter + chimeric intron + Kozak sequence)

Enrichment of transfected cells

-

GFP & Puromycin (pTAL7A) Blasticidin (pTAL7B)

In-vitro transcription

-

T7 promoter

Codon usage

Not optimized for mammalian cells

Optimized for mammalian cells

Tag

-

Flag tag