pTAL4 | pTAL7 | |
---|---|---|
Site for GoldenGate TALEN Assembly | Esp3I | Esp3I |
Bacterial Resistance | Ampicillin | Kanamycin |
Screening for successful cloning | LacZ | LacZ |
TALEN architecture | Full length Voytas et al. [13] | Truncated C- & N-termini similar to Miller et al. [6] |
FokI nuclease | Wild-type | Wild-type |
Expression system | Yeast | Mammalian cells (CAG promoter + chimeric intron + Kozak sequence) |
Enrichment of transfected cells | - | GFP & Puromycin (pTAL7A) Blasticidin (pTAL7B) |
In-vitro transcription | - | T7 promoter |
Codon usage | Not optimized for mammalian cells | Optimized for mammalian cells |
Tag | - | Flag tag |