Expression profiles in selected biotic stress responsive unigenes. Quantitative real-time PCRs were performed to analyze expression in candidate unigenes in Calcutta 4 mRNA from pooled timepoints of 3, 6 and 9DAI, with triplicate samples of each. Expression analysis was conducted for LRR receptor-like serine/threonine-protein kinase RGA genes and SSR-containing defence-related unigenes. Normalization of expression was performed using an Elongation Factor gene for M. acuminata as reference. Bars represent the standard error of the mean of technical replicates for each biological sample.