Table 3 The primer efficiency, r 2 -value, dynamic range and limit of detection of the Gut Microbiotassay
Primer set | Efficiency, % | r 2 | Dynamic range | Limit of detection, ng/μl |
|---|---|---|---|---|
Domain Bacteria B V4-V5 | 75-106 | >0.968 | 4-6-fold | 0.05-0.0005 |
Phylum Firmicutes* | 86-97 | >0.994 | 3-5-fold | 0.05-0.005 |
Phylum Bacilli | 99-101 | >0.943 | 4-5-fold | 0.05-0.005 |
Genus Enterococcus | 87 | >0.998 | 5-fold | 0.005 |
Genus Lactobacillus | 112 | >0.992 | 5-fold | 0.005 |
Genus Streptococcus | 102 | >0.999 | 5-fold | 0.005 |
Family Clostridium cluster I | 96 | >0.995 | 4-fold | 0.05 |
Species Clostridium perfringens | 87 | >0.999 | 5-fold | 0.005 |
Family Clostridium cluster IV | 103 | >0.997 | 5-fold | 0.005 |
Family Clostridium cluster XIV | 96 | >0.999 | 4-fold | 0.05 |
Phylum Bacteroidetes | 91 | >0.991 | 6-fold | 0.0005 |
Genus Bacteroides | 85 | >0.997 | 5-fold | 0.005 |
Phylum Actinobacteria | 92 | >0.988 | 6-fold | 0.0005 |
Family Bifidobacteriaceae | 77 | >0.995 | 5-fold | 0.005 |
Class β- and γ-proteobacteria | 87 | >0.999 | 5-fold | 0.005 |
Family Enterobacteriacea | 84 | >0.996 | 5-fold | 0.005 |
Species Escherichia coli | 91 | >0.999 | 6-fold | 0.0005 |
Class ϵ-proteobacteria | 86 | >0.995 | 6-fold | 0.0005 |
Class δ-proteobacteria | 91 | >0.995 | 5-fold | 0.005 |
Phylum Fusobacteria | 85 | >0.997 | 4-fold | 0.005 |
Phylum Verrucomicrobia | 107 | >0.990 | 6-fold | 0.0005 |
Phylum Spirochaetes | 80 | >0.996 | 5-fold | 0.005 |
Domain Archaea | 95 | >0.996 | 5-fold | 0.005 |