Figure 5

Integration of L. angustifolius genetic and physical maps: assignment of linkage groups (NLLs) to corresponding chromosomes by BAC-FISH. A: Localization of two BAC clones 074I10 (red signals) and 055L12 (green signals) in two different chromosomes. BAC 074I10 position is represented by genetic marker 074I10_3_1, mapped in NLL-07 (see Results and Discussion). B: Genetic marker 004G15_3 (generated from BAC 004G15, red signals) mapped in NLL-05. C: Three genetic markers (076K16_5, 076K16_3F3 and 080B11_3), generated from BAC clones 076K16 (green signals) and 080B11 (red signals), mapped in NLL-06 and co-localized in the same chromosome site (overlapping signals). Yellow colored genetic markers on NLL-06 graph gave dispersed BAC-FISH signals and are not shown in this figure. D: Two genetic markers 077C13_3 (green) and 057K22_3F2 (red) mapped in NLL-10 and corresponding BACs co-localized in the same chromosome site (yellow signals). E: BAC 083C06 (green signals) co-localized in the same chromosome arm as BAC 017B07 (red signals) previously mapped in linkage group NLL-20. BAC clone DNA labeled with tetramethylrhodamine-5-dUTP (red signals) and digoxigenin-11-dUPT (green signals), chromosomes counterstained with DAPI. Overlapping signals - yellow. Scale bar = 5 μm.