Function of the Q rich region of the C-terminal end of Sry. A) Regulation of the AR600 promoter construct by Sryα (blue), Sryβ (red) and Sryγ (green). Multiple mutations made to Sryα (B), Sryβ (C) or Sryγ (D) that altered AR600 promoter regulation. For Sryα three deletion constructs were created (B): Sryα(del) removing 13 amino acids in the Q-rich region making it more similar to Sryβ, Sryα(−QR) removing the whole 25 Q-rich region, and Sryα(HMGbox) containing only the HMG box of Sryα . For Sryβ two alternative constructs were created (C): Sryβ(+QR) adding the 13 amino acids of Sryα in the Q-rich region missing from Sryβ, Sryβ(−QR) removing the entire 25 amino acids of the Q-rich region. Two alternative constructs were created for Sryγ (D): Sryγ(del) removing 13 amino acids in the Q-rich region making it more similar to Sryβ, Sryγ(−QR) removing the whole 25 Q-rich region. Error bars represent the standard error of the mean and significance is shown as ∗ = p ≤ 0.05, ∗∗ = p ≤ 0.01, ∗∗∗ = p ≤ 0.001 for each compared to the control (black).