HMG box variations in the rat Sry proteins. A) Amino acid variations (red) found in the HMG box of rat Sry proteins shown on a model of Sry1 (gray) when bound to DNA (cyan) based on the known human structure. B) Averaged movement during molecular dynamic simulations for models of Sryα interacting with DNA with each mutation from A. Shown as the averaged carbon alpha RMSD for the structure over a 1 nanosecond simulation. C) Predicted binding energy to DNA of the energy minimized structures of Sryα or containing one of the four mutations in Sryα.