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Table 1 Summary of T. salsuginea ESTs generated from 454 sequencing and assembled contigs

From: Transcriptome sequencing and microarray design for functional genomics in the extremophile Arabidopsis relative Thellungiella salsuginea (Eutrema salsugineum)

Library Non-normalized Normalized Combined1
Number of reads 811,683 400,631 1,212,314
Number of contaminated reads2 4,496 2,506 7,002
Average read length (bp) 566 257 464
Total reads (Mbp) 459.54 103.13 562.67
Number of assembled reads 712,262 376,509 1,060,666
Number of contigs3 33,870 28,928 46,220
Number of contigs with only 454 reads4 21,662 16,949 33,147
Average coverage 11.99 6.74 12.63
N50 (bp) 665 632 646
Average contig length (bp) 621 502 567
Number of contigs with ORF5 33,625 28,416 45,583
Number of Arabidopsis peptides found6 23,787 23,220 24,457
  1. 1Combined 454 reads derived from normalized and non-normalized library.
  2. 2Best hits with E-value < 1e-10, non streptophyta sequences (NCBI, nr, nt, as of February 12, 2010).
  3. 3Includes publicly available T. salsuginea ESTs (as of April 19, 2010).
  4. 4Number of contigs minus number of contigs assembled with at least one publicly available EST.
  5. 5Open reading frames (ORFs) predicted including partial ORFs (Min et al. [83]).
  6. 6Any hits with E-value < 0.001 from BLASTX search against TAIR9 database (27,379 peptides).