Figure 3

The potential binding sites of selected targets predicted for 5 novel cabbage miRNAs. Figure describes binding sites in the selected targets of five newly identified miRNAs: bol-miR_new4, bol-miR_new17 and also the experimentally confirmed bol-miR9408, bol-miR9409 and bol-miR9411. MiRNAs targets are represented by black line with their contig ID identifier, sequence length and annotation. Blue box indicates the miRNA binding site (exact coordinates included). The miRNA:mRNA sequence alignments, together with the calculated score and minimum free energy of the duplex, are presented beneath each target binding site. In the alignment “|” refers to perfect complementary between bases, “-“ represents the gap, while “:” stands for G:U wobble pair. Above each of the presented miRNAs target (contig) the scheme of the A. thaliana homologous cDNA sequence is placed (red line). The circle indicates the referring miRNA:mRNA binding site (exact coordinates included).