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Table 4 Gene expression values and the predicted autosomal interactions for the selected genes linked to pyrethroid resistance in Musca domestica as assayed by qPCR

From: A whole transcriptomal linkage analysis of gene co-regulation in insecticide resistant house flies, Musca domestica

SCOPfunctional annotation Predicted gene function Accession number Relative gene expression ± SE
General Detailed Pfam annotation§ Gene ALHF A2345 A1345 A1245 A1235 A1234
Metabolism Other Carboxylesterase ALHF_03407.g2111 3.1 ± 0.2 2.4 ± 0.1 1.8 ± 0.12* 3.0 ± 0.1 2.9 ± 0.1 1.1 ± 0.11*
    ALHF_05628.g3847 6.6 ± 1.3 2.1 ± 0.3* 1.0 ± 0.1* 4.0 ± 0.4 3.2 ± 0.1 4.4 ± 0.8
    ALHF_00771.g422 26 ± 5.5 14 ± 1.2 0.24 ± 0.07* 18 ± 1.5 26 ± 2 3.1 ± 0.3*
  Redox Cytochrome P450 ALHF_04553.g3033 2.7 ± 0.09 2.5 ± 0.1 2.7 ± 0.2 2.5 ± 0.08 3.0 ± 0.2 0.8 ± 0.03*
    ALHF_05265.g3608 510 ± 28 300 ± 5.0 15 ± 2.6* 530 ± 16 500 ± 18 300 ± 4.3
    ALHF_03088.g1882 310 ± 9.3 310 ± 10 190 ± 9.0 150 ± 4.4 310 ± 18 120 ± 6.4*
    ALHF_02791.g1651 12 ± 0.2 7.4 ± 0.7 7.9 ± 0.04 8.8 ± 0.2 13 ± 0.9 2.4 ± 0.09*
    ALHF_07553.g4857 6.2 ± 0.6 2.6 ± 0.4* 3.1 ± 0.6* 9.5 ± 2.3 6.2 ± 0.5 2.6 ± 0.3*
    ALHF_04445.g2939 3.0 ± 0.1 1.6 ± 0.2* 1.8 ± 0.4* 4.0 ± 0.4 3.0 ± 0.04 1.0 ± 0.2*
    ALHF_04444.g2938 3.6 ± 0.06 1.9 ± 0.1 2.1 ± 0.09 5.6 ± 2.1 3.6 ± 0.1 1.0 ± 0.2*
    ALHF_03006.g1816 2.3 ± 0.09 1.9 ± 0.3 1.1 ± 0.08* 3.8 ± 0.4 2.1 ± 0.06 0.9 ± 0.1*
    ALHF_01822.g1025 4.3 ± 1.0 1.3 ± 0.2* 1.9 ± 0.4* 5.6 ± 1.2 4.2 ± 0.2 0.6 ± 0.1*
    ALHF_04730.g3176 2.1 ± 0.02 1.2 ± 0.2 2.6 ± 0.3 7.1 ± 2.7 1.8 ± 0.09 0. 7 ± 0.09*
    ALHF_03063.g1860†† - - - - - -
    ALHF_05136.g3505 2.4 ± 0.3 0.9 ± 0.1* 0.4 ±0.2* 3.2 ± 0.4 2.5 ± 0.2 1.5 ± 0.1
    ALHF_07623.g4891 1.9 ± 0.2 1.8 ± 0.2 0.4 ± 0.2* 4.6 ± 1.2 2.2 ± 0.1 1.0 ± 0.1 *
    ALHF_08221.g5182 2.6 ± 0.2 1.6 ± 0.04 1.8 ± 0.3 0.6 ±0.04 2.9 ± 0.04 0.6 ± 0.05*
    ALHF_04665.g3125 2.9 ± 0.08 1.0 ± 0.05* 1.1 ± 0.06* 0.8 ±0.03* 2.8 ± 0.09 1.0 ± 0.03*
    ALHF_01339.g731 2.0 ± 0.08 2.3 ± 0.07 0.2 ± 0.03* 1.1 ± 0.03 2.1 ± 0.07 0.8 ± 0.04*
    ALHF_04736.g3182†† - - - - - -
    ALHF_03849.g2446†† - - - - - -
   Glutathione-S-transferase ALHF_04900.g3328 2.8 ± 0.4 2.1 ± 0.2 0.9 ± 0.08* 2.3 ± 0.2 2.8 ± 0.2 2.2 ± 0.2
    ALHF_04476.g2964 2.4 ± 0.2 1.7 ± 0.2 0.6 ±0.07* 1.9 ± 0.2 1.8 ± 0.08 0.7 ± 0.02*
    ALHF_03731.g2351 2.4 ± 0.3 1.8 ± 0.3 0.4 ± 0.03* 1.2 ± 0.08 1.5 ± 0.07 0.6 ± 0.08*
    ALHF_04477.g2965 1.5 ± 0.02 1.1 ± 0.11 0.6 ± 0.07* 1.7 ± 0.1 1.3 ± 0.06 0.8 ± 0.1*
    ALHF_03145.g1917 1.9 ± 0.2 0.7 ± 0.06* 0.5 ± 0.01* 1.6 ± 0.2 1.8 ± 0.06 0.9 ± 0.02*
Regulation Kinase / phosphatase Protein kinase domain ALHF_02546.g1487 2.9 ± 0.09 3.8 ± 0.75 0.4 ± 0.04* 2.2 ± 0.3 2.6 ± 0.2 0.5 ± 0.08*
    ALHF_00685.g381 3.6 ± 0.4 3.2 ± 0.7 0.8 ±0.2* 1.1 ± 0.09* 3.3 ± 0.3 0.5 ± 0.04*
    ALHF_03462.g2147 3.7 ± 0.7 4.1 ± 0.4 0.5 ± 0.01* 1.5 ± 0.2* 3.5 ± 0.2 0.2 ± 0.2*
    ALHF_02885.g1722 1.8 ± 0.2 1.8 ± 0.2 0.6 ±0.2* 1.5 ± 0.8 1.6 ± 0.1 0.8 ±0.1*
    ALHF_00823.g452 2.0 ± 0.2 1.8 ± 0.2 0.8 ± 0.2* 1.2 ± 0.09 1.8 ± 0.2 0.7 ± 0.1 *
    ALHF_04500.g2986 1.7 ± 0.09 1.1 ± 0.04 0.7 ± 0.2* 1.5 ± 0.1 1.7 ± 0.04 0.9 ± 0.06*
    ALHF_04095.g2646 1.7 ± 0.1 1.1 ± 0.06 0.9 ±0.2 1.0 ± 0.08 1.5 ± 0.03 1.0 ± 0.2
    ALHF_01595.g882 2.3 ± 0.2 0.8 ± 0.06* 0.8 ± 0.1* 1.4 ± 0.2 2.5 ± 0.2 1.0 ± 0.1*
    ALHF_01832.g1033 2.3 ± 0.2 2.8 ± 0.6 0.4 ± 0.09* 1.0 ± 0.06* 1.9 ± 0.1 0.4 ± 0.06*
    ALHF_08078.g5122 2.6 ± 0.7 1.0 ± 0.04 0.5 ± 0.03* 1.7 ± 0.1 2.4 ± 0.2 0.6 ± 0.06*
    ALHF_11277.g6269 2.3 ± 0.1 0.7 ± 0.05* 0.2 ± 0.02* 1.8 ± 0.1 2.1 ± 0.2 0.8 ± 0.2*
    ALHF_11442.g6384†† - - - - - -
    ALHF_00727.g395†† - - - - - -
   Protein tyrosine kinase ALHF_11829.g6650 1.7 ± 0.08 1.3 ± 0.09 1.5 ± 0.17 2.2 ± 0.4 1.9 ± 0.1 1.3 ± 0.1
    ALHF_11144.g6194 4.0 ± 0.8 3.2 ± 0.2 1.4 ± 0.2* 2.5 ± 0.7 5.1 ± 0.2 0.8 ± 0.04*
    ALHF_09312.g5609 1.9 ± 0.1 1.3 ± 0.1 0.7 ± 0.1* 1.7 ± 0.2 1.7 ± 0.06 0.7 ± 0.1*
    ALHF_10712.g5974 1.8 ± 0.1 0.7 ± 0.04* 0.6 ± 0.2* 1.7 ± 0.3 1.8 ± 0.08 0.7 ± 0.1*
    ALHF_07173.g4665 1.5 ± 0.1 1.3 ± 0.1 0.8 ± 0.05* 1.2 ± 0.2 1.6 ± 0.07 0.5 ± 0.1*
    ALHF_03649.g2289 2.1 ± 0.1 0.9 ± 0.07* 0.4 ± 0.2* 1.5 ± 0.2 2.1 ± 0.2 1.1 ± 0.06*
    ALHF_05773.g3933†† - - - - - -
    ALHF_11245.g6252 1.7 ± 0.07 0.6 ± 0.03* 0.2 ± 0.06* 0.5 ± 0.2* 1.9 ± 0.09 0.9 ± 0.09*
   Protein-tyrosine phosphatase ALHF_11768.g6612 39 ± 4.9 1.6 ± 0.07* 0.6 ± 0.05* 0.4 ± 0.1* 36 ± 3.8 0.5 ± 0.03*
    ALHF_03863.g2457 1.5 ± 0.06 0.9 ± 0.09 0.5 ± 0.04* 1.2 ± 0.2 1.7 ± 0.06 0.9 ± 0.08*
  Signal transduction GPCR (rhodopsin family) ALHF_01760.g986 1.6 ± 0.09 1.0 ± 0.08 0.9 ± 0.1* 1.1 ± 0.03 1.7 ± 0.1 1.0 ± 0.1
    ALHF_02400.g1393 1.7 ± 0.07 0.9 ± 0.07 0.7 ± 0.05* 1.2 ± 0.2 1.6 ± 0.1 0.9 ± 0.1*
    ALHF_06811.g4468 1.7 ± 0.1 0.9 ± 0.03* 0.6 ± 0.07* 1.2 ± 0.2 1.7 ± 0.1 0.8 ± 0.08*
    ALHF_07519.g4838 1.7 ± 0.07 1.1 ± 0.09 0.2 ± 0.06* 0.9 ± 0.2 1.6 ± 0.06 0.9 ± 0.04
    ALHF_02706.g1581 1.4 ± 0.1 0.52 ± 0.1* 0.37 ± 0.1* 1.2 ± 0.3 1.3 ± 0.04 1.2 ± 0.11
    ALHF_04422.g2918†† - - - - - -
   Adenylate and Guanylate cyclase catalytic domain ALHF_01050.g580 2.6 ± 0.3 1.3 ± 0.1 0.8 ± 0.09* 1.1 ± 0.2* 2.2 ± 0.21 1.3 ± 0.3*
    ALHF_07748.g4948 2.1 ± 0.2 1.2 ± 0.1 0.6 ± 0.05* 1.3 ± 0.2 1.8 ± 0.05 1.0 ± 0.2*
   Serpentine type 7TM GPCR chemoreceptor Srw ALHF_01902.g1074 - - - - - -
Intra-cellular processes Proteases Serpins ALHF_07374.g4763 4.5 ± 0.8 1.8 ± 0.2 0.6 ± 0.2* 1.3 ± 0.1* 2.3 ± 0.2 0.5 ± 0.08*
    ALHF_01182.g646 1.7 ± 0.1 0.8 ± 0.07 0.3 ± 0.07* 1.1 ± 0.08 1.8 ± 0.1 0.7 ± 0.08*
   Carboxypeptidases ALHF_04057.g2616 2.2 ± 0.3 1.5 ± 0.1 0.5 ± 0.1* 1.3 ± 0.2 1.8 ± 0.07 0.9 ± 0.1*
    ALHF_05871.g3981†† - - - - - -
   Subtilase ALHF_00530.g295†† - - - - - -
   Aspartyl protease ALHF_06529.g4317 2.5 ± 0.1 2.3 ± 0.2 1.4 ± 0.2* 1.3 ± 0.1* 2.1 ± 0.1 1.4 ± 0.01*
   Peptidases ALHF_00761.g417 780 ± 50 530 ± 100 630 ± 60 23 ± 2* 650 ± 110 530 ± 20
    ALHF_03218.g1970 29 ± 2.1 30 ± 3 35 ± 5 2.6 ± 0.4* 17 ± 3* 8.2 ± 1*
    ALHF_02207.g1267 145 ± 10 150 ± 20 120 ± 3 5.0 ± 0.3* 4.6 ± 2* 4.9 ± 0.6*
    ALHF_07511.g4836 2.1 ± 0.1 1.5 ± 0.2 0.52 ± 0.04* 3.2 ± 0.2 0.52 ± 0.07* 0.41 ± 0.08*
    ALHF_01861.g1049†† - - - - - -
    ALHF_05334.g3663 1.4 ± 0.04 1.0 ± 0.1 0.66 ± 0.06* 1.5 ± 0.1 0.75 ± 0.07* 0.40 ± 0.1*
  1. Structural Classification of Proteins (SCOP) http://supfam.cs.bris.ac.uk/SUPERFAMILY/.
  2. ††Autosomal interactions are named after the combination of the autosomes donated by the aabys strain in each case where a M. domestica autosomal combination line had a level of gene expression significantly lower than the ALHF strain at the α = 0.05 level of significance.
  3. ††Gene was not differentially-expressed between the aabys and ALHF strains.
  4. §Gene predicted function as evidenced by the Pfam HMM-A homology (http://pfam.sanger.ac.uk/).
  5. *Indicates that the gene expression value within a given M. domestica autosomal line was significantly lower than the expression in the parental ALHF strain at the α = 0.05 level of significance.