Skip to main content

Table 4 Gene expression values and the predicted autosomal interactions for the selected genes linked to pyrethroid resistance in Musca domestica as assayed by qPCR

From: A whole transcriptomal linkage analysis of gene co-regulation in insecticide resistant house flies, Musca domestica

SCOP†functional annotation

Predicted gene function

Accession number

Relative gene expression ± SE

General

Detailed

Pfam annotation§

Gene

ALHF

A2345

A1345

A1245

A1235

A1234

Metabolism

Other

Carboxylesterase

ALHF_03407.g2111

3.1 ± 0.2

2.4 ± 0.1

1.8 ± 0.12*

3.0 ± 0.1

2.9 ± 0.1

1.1 ± 0.11*

   

ALHF_05628.g3847

6.6 ± 1.3

2.1 ± 0.3*

1.0 ± 0.1*

4.0 ± 0.4

3.2 ± 0.1

4.4 ± 0.8

   

ALHF_00771.g422

26 ± 5.5

14 ± 1.2

0.24 ± 0.07*

18 ± 1.5

26 ± 2

3.1 ± 0.3*

 

Redox

Cytochrome P450

ALHF_04553.g3033

2.7 ± 0.09

2.5 ± 0.1

2.7 ± 0.2

2.5 ± 0.08

3.0 ± 0.2

0.8 ± 0.03*

   

ALHF_05265.g3608

510 ± 28

300 ± 5.0

15 ± 2.6*

530 ± 16

500 ± 18

300 ± 4.3

   

ALHF_03088.g1882

310 ± 9.3

310 ± 10

190 ± 9.0

150 ± 4.4

310 ± 18

120 ± 6.4*

   

ALHF_02791.g1651

12 ± 0.2

7.4 ± 0.7

7.9 ± 0.04

8.8 ± 0.2

13 ± 0.9

2.4 ± 0.09*

   

ALHF_07553.g4857

6.2 ± 0.6

2.6 ± 0.4*

3.1 ± 0.6*

9.5 ± 2.3

6.2 ± 0.5

2.6 ± 0.3*

   

ALHF_04445.g2939

3.0 ± 0.1

1.6 ± 0.2*

1.8 ± 0.4*

4.0 ± 0.4

3.0 ± 0.04

1.0 ± 0.2*

   

ALHF_04444.g2938

3.6 ± 0.06

1.9 ± 0.1

2.1 ± 0.09

5.6 ± 2.1

3.6 ± 0.1

1.0 ± 0.2*

   

ALHF_03006.g1816

2.3 ± 0.09

1.9 ± 0.3

1.1 ± 0.08*

3.8 ± 0.4

2.1 ± 0.06

0.9 ± 0.1*

   

ALHF_01822.g1025

4.3 ± 1.0

1.3 ± 0.2*

1.9 ± 0.4*

5.6 ± 1.2

4.2 ± 0.2

0.6 ± 0.1*

   

ALHF_04730.g3176

2.1 ± 0.02

1.2 ± 0.2

2.6 ± 0.3

7.1 ± 2.7

1.8 ± 0.09

0. 7 ± 0.09*

   

ALHF_03063.g1860††

-

-

-

-

-

-

   

ALHF_05136.g3505

2.4 ± 0.3

0.9 ± 0.1*

0.4 ±0.2*

3.2 ± 0.4

2.5 ± 0.2

1.5 ± 0.1

   

ALHF_07623.g4891

1.9 ± 0.2

1.8 ± 0.2

0.4 ± 0.2*

4.6 ± 1.2

2.2 ± 0.1

1.0 ± 0.1 *

   

ALHF_08221.g5182

2.6 ± 0.2

1.6 ± 0.04

1.8 ± 0.3

0.6 ±0.04

2.9 ± 0.04

0.6 ± 0.05*

   

ALHF_04665.g3125

2.9 ± 0.08

1.0 ± 0.05*

1.1 ± 0.06*

0.8 ±0.03*

2.8 ± 0.09

1.0 ± 0.03*

   

ALHF_01339.g731

2.0 ± 0.08

2.3 ± 0.07

0.2 ± 0.03*

1.1 ± 0.03

2.1 ± 0.07

0.8 ± 0.04*

   

ALHF_04736.g3182††

-

-

-

-

-

-

   

ALHF_03849.g2446††

-

-

-

-

-

-

  

Glutathione-S-transferase

ALHF_04900.g3328

2.8 ± 0.4

2.1 ± 0.2

0.9 ± 0.08*

2.3 ± 0.2

2.8 ± 0.2

2.2 ± 0.2

   

ALHF_04476.g2964

2.4 ± 0.2

1.7 ± 0.2

0.6 ±0.07*

1.9 ± 0.2

1.8 ± 0.08

0.7 ± 0.02*

   

ALHF_03731.g2351

2.4 ± 0.3

1.8 ± 0.3

0.4 ± 0.03*

1.2 ± 0.08

1.5 ± 0.07

0.6 ± 0.08*

   

ALHF_04477.g2965

1.5 ± 0.02

1.1 ± 0.11

0.6 ± 0.07*

1.7 ± 0.1

1.3 ± 0.06

0.8 ± 0.1*

   

ALHF_03145.g1917

1.9 ± 0.2

0.7 ± 0.06*

0.5 ± 0.01*

1.6 ± 0.2

1.8 ± 0.06

0.9 ± 0.02*

Regulation

Kinase / phosphatase

Protein kinase domain

ALHF_02546.g1487

2.9 ± 0.09

3.8 ± 0.75

0.4 ± 0.04*

2.2 ± 0.3

2.6 ± 0.2

0.5 ± 0.08*

   

ALHF_00685.g381

3.6 ± 0.4

3.2 ± 0.7

0.8 ±0.2*

1.1 ± 0.09*

3.3 ± 0.3

0.5 ± 0.04*

   

ALHF_03462.g2147

3.7 ± 0.7

4.1 ± 0.4

0.5 ± 0.01*

1.5 ± 0.2*

3.5 ± 0.2

0.2 ± 0.2*

   

ALHF_02885.g1722

1.8 ± 0.2

1.8 ± 0.2

0.6 ±0.2*

1.5 ± 0.8

1.6 ± 0.1

0.8 ±0.1*

   

ALHF_00823.g452

2.0 ± 0.2

1.8 ± 0.2

0.8 ± 0.2*

1.2 ± 0.09

1.8 ± 0.2

0.7 ± 0.1 *

   

ALHF_04500.g2986

1.7 ± 0.09

1.1 ± 0.04

0.7 ± 0.2*

1.5 ± 0.1

1.7 ± 0.04

0.9 ± 0.06*

   

ALHF_04095.g2646

1.7 ± 0.1

1.1 ± 0.06

0.9 ±0.2

1.0 ± 0.08

1.5 ± 0.03

1.0 ± 0.2

   

ALHF_01595.g882

2.3 ± 0.2

0.8 ± 0.06*

0.8 ± 0.1*

1.4 ± 0.2

2.5 ± 0.2

1.0 ± 0.1*

   

ALHF_01832.g1033

2.3 ± 0.2

2.8 ± 0.6

0.4 ± 0.09*

1.0 ± 0.06*

1.9 ± 0.1

0.4 ± 0.06*

   

ALHF_08078.g5122

2.6 ± 0.7

1.0 ± 0.04

0.5 ± 0.03*

1.7 ± 0.1

2.4 ± 0.2

0.6 ± 0.06*

   

ALHF_11277.g6269

2.3 ± 0.1

0.7 ± 0.05*

0.2 ± 0.02*

1.8 ± 0.1

2.1 ± 0.2

0.8 ± 0.2*

   

ALHF_11442.g6384††

-

-

-

-

-

-

   

ALHF_00727.g395††

-

-

-

-

-

-

  

Protein tyrosine kinase

ALHF_11829.g6650

1.7 ± 0.08

1.3 ± 0.09

1.5 ± 0.17

2.2 ± 0.4

1.9 ± 0.1

1.3 ± 0.1

   

ALHF_11144.g6194

4.0 ± 0.8

3.2 ± 0.2

1.4 ± 0.2*

2.5 ± 0.7

5.1 ± 0.2

0.8 ± 0.04*

   

ALHF_09312.g5609

1.9 ± 0.1

1.3 ± 0.1

0.7 ± 0.1*

1.7 ± 0.2

1.7 ± 0.06

0.7 ± 0.1*

   

ALHF_10712.g5974

1.8 ± 0.1

0.7 ± 0.04*

0.6 ± 0.2*

1.7 ± 0.3

1.8 ± 0.08

0.7 ± 0.1*

   

ALHF_07173.g4665

1.5 ± 0.1

1.3 ± 0.1

0.8 ± 0.05*

1.2 ± 0.2

1.6 ± 0.07

0.5 ± 0.1*

   

ALHF_03649.g2289

2.1 ± 0.1

0.9 ± 0.07*

0.4 ± 0.2*

1.5 ± 0.2

2.1 ± 0.2

1.1 ± 0.06*

   

ALHF_05773.g3933††

-

-

-

-

-

-

   

ALHF_11245.g6252

1.7 ± 0.07

0.6 ± 0.03*

0.2 ± 0.06*

0.5 ± 0.2*

1.9 ± 0.09

0.9 ± 0.09*

  

Protein-tyrosine phosphatase

ALHF_11768.g6612

39 ± 4.9

1.6 ± 0.07*

0.6 ± 0.05*

0.4 ± 0.1*

36 ± 3.8

0.5 ± 0.03*

   

ALHF_03863.g2457

1.5 ± 0.06

0.9 ± 0.09

0.5 ± 0.04*

1.2 ± 0.2

1.7 ± 0.06

0.9 ± 0.08*

 

Signal transduction

GPCR (rhodopsin family)

ALHF_01760.g986

1.6 ± 0.09

1.0 ± 0.08

0.9 ± 0.1*

1.1 ± 0.03

1.7 ± 0.1

1.0 ± 0.1

   

ALHF_02400.g1393

1.7 ± 0.07

0.9 ± 0.07

0.7 ± 0.05*

1.2 ± 0.2

1.6 ± 0.1

0.9 ± 0.1*

   

ALHF_06811.g4468

1.7 ± 0.1

0.9 ± 0.03*

0.6 ± 0.07*

1.2 ± 0.2

1.7 ± 0.1

0.8 ± 0.08*

   

ALHF_07519.g4838

1.7 ± 0.07

1.1 ± 0.09

0.2 ± 0.06*

0.9 ± 0.2

1.6 ± 0.06

0.9 ± 0.04

   

ALHF_02706.g1581

1.4 ± 0.1

0.52 ± 0.1*

0.37 ± 0.1*

1.2 ± 0.3

1.3 ± 0.04

1.2 ± 0.11

   

ALHF_04422.g2918††

-

-

-

-

-

-

  

Adenylate and Guanylate cyclase catalytic domain

ALHF_01050.g580

2.6 ± 0.3

1.3 ± 0.1

0.8 ± 0.09*

1.1 ± 0.2*

2.2 ± 0.21

1.3 ± 0.3*

   

ALHF_07748.g4948

2.1 ± 0.2

1.2 ± 0.1

0.6 ± 0.05*

1.3 ± 0.2

1.8 ± 0.05

1.0 ± 0.2*

  

Serpentine type 7TM GPCR chemoreceptor Srw

ALHF_01902.g1074

-

-

-

-

-

-

Intra-cellular processes

Proteases

Serpins

ALHF_07374.g4763

4.5 ± 0.8

1.8 ± 0.2

0.6 ± 0.2*

1.3 ± 0.1*

2.3 ± 0.2

0.5 ± 0.08*

   

ALHF_01182.g646

1.7 ± 0.1

0.8 ± 0.07

0.3 ± 0.07*

1.1 ± 0.08

1.8 ± 0.1

0.7 ± 0.08*

  

Carboxypeptidases

ALHF_04057.g2616

2.2 ± 0.3

1.5 ± 0.1

0.5 ± 0.1*

1.3 ± 0.2

1.8 ± 0.07

0.9 ± 0.1*

   

ALHF_05871.g3981††

-

-

-

-

-

-

  

Subtilase

ALHF_00530.g295††

-

-

-

-

-

-

  

Aspartyl protease

ALHF_06529.g4317

2.5 ± 0.1

2.3 ± 0.2

1.4 ± 0.2*

1.3 ± 0.1*

2.1 ± 0.1

1.4 ± 0.01*

  

Peptidases

ALHF_00761.g417

780 ± 50

530 ± 100

630 ± 60

23 ± 2*

650 ± 110

530 ± 20

   

ALHF_03218.g1970

29 ± 2.1

30 ± 3

35 ± 5

2.6 ± 0.4*

17 ± 3*

8.2 ± 1*

   

ALHF_02207.g1267

145 ± 10

150 ± 20

120 ± 3

5.0 ± 0.3*

4.6 ± 2*

4.9 ± 0.6*

   

ALHF_07511.g4836

2.1 ± 0.1

1.5 ± 0.2

0.52 ± 0.04*

3.2 ± 0.2

0.52 ± 0.07*

0.41 ± 0.08*

   

ALHF_01861.g1049††

-

-

-

-

-

-

   

ALHF_05334.g3663

1.4 ± 0.04

1.0 ± 0.1

0.66 ± 0.06*

1.5 ± 0.1

0.75 ± 0.07*

0.40 ± 0.1*

  1. †Structural Classification of Proteins (SCOP) http://supfam.cs.bris.ac.uk/SUPERFAMILY/.
  2. ††Autosomal interactions are named after the combination of the autosomes donated by the aabys strain in each case where a M. domestica autosomal combination line had a level of gene expression significantly lower than the ALHF strain at the α = 0.05 level of significance.
  3. ††Gene was not differentially-expressed between the aabys and ALHF strains.
  4. §Gene predicted function as evidenced by the Pfam HMM-A homology (http://pfam.sanger.ac.uk/).
  5. *Indicates that the gene expression value within a given M. domestica autosomal line was significantly lower than the expression in the parental ALHF strain at the α = 0.05 level of significance.
Back to article page

BMC Genomics

ISSN: 1471-2164

Contact us