Figure 2

The effects of NtTTG2 silencing on flower and seed development. (a) Flowers at five development stages (S1-S5) from 120-d-old WT/RFP and 160-d-old TTG2RNAi4 plants. (b, c) Flower anthocyanin extracts and spectrophotometric analyses. The floral anthocyanin content was quantified as relative unit (r.u.). (d) Real-time RT-PCR analyses of floral transcripts of the NtTTG2 gene as well as the DFR and ANS genes, which are involved in the late stage of the anthocyanin biosynthesis pathway. (e) Growing fruits (left) from 140-d-old WT/RFP and 170-d-old TTG2RNAi4, and mature fruits (right) from 170-d-old WT/RFP and 210-d-old TTG2RNAi4 plants. (f) The number of seeds per mature fruit. Data were given as mean values ± SD bars of results from three (c, d) and five (f) experimental repeats (30 fruits/repeat). Significant levels (*P < 0.05; **P < 0.01) in differences between corresponding data pairs were analyzed by one-way ANOVA and LSD test.