Figure 4

FGSG_03333 encodes a putative phosphorylcholine phosphatase with a shared selection process with bacterial plant pathogens. For protein FGSG_03333, a BLASTp search returns 231 hits with E-value < 1.0e-05 and these were merged with our results from the MycoCosm tree. A MUSCLE alignment [48] and maximum likelihood phylogenetic tree estimation by PhyML [49] returns this phylogenetic tree with branch support values, visualized by FigTree [50]. The tree indicates that this protein has been selectively retained in hemibiotrophic and necrotrophic fungal pathogens of cereals, plants and insects. Furthermore, this protein is closely related to a highly conserved protein in a large number of Pseudomonads, indicating a shared selection process and suggesting a common role in pathogenicity.