Table 1 The top 10 pathogen-associated F. graminearum proteins in terms of cereal and pathogen F-measure
Protein id | F-measure | Pfam domain (E-value, ID) | Phyre2 structure (Confidence/Coverage) | Molecular weight (kDA) | Signal peptide | Distribution of significant NR phmmer hits |
|---|---|---|---|---|---|---|
| Â | Cereal | Â | Â | Â | Â | Â |
FGSG_03333 | 0.71 | Haloacid dehalogenase-like hydrolase (8e-10, PF12710) | Phosphorylcholine phosphatase (100%/90%) | 41.1 | Yes | 93% Bacteria, 7% Ascomycota |
FGSG_03338 | 0.7 | – | Serine protease inhibitor 1 (98.4%/52%) | 14.4 | No | 100% Ascomycota |
FGSG_09148 | 0.69 | Peptidase C69 (3.1e-25, PF03577) | Acyl-coenzyme (99.2%/44%) | 57.2 | Yes | 90% Bacteria, 9% Eukaryota, 1% Archaea |
FGSG_03339 | 0.66 | – | Serine protease inhibitor 1 (99.2%/55%) | 14.7 | No | 100% Ascomycota |
FGSG_09328 | 0.66 | Fungal specific transcription factor domain (3.5e-05, PF04082) | Centromere DNA-binding protein complex cbf3 (98.2%/72%) | 62.6 | No | 94% Ascomycota, 6% Basidiomycota |
FGSG_04015 | 0.66 | – | – | 57.4 | No | 100% Ascomycota |
FGSG_03861 | 0.64 | DUF3425 (1.8e-14, PF11095) | Pyrimidine pathway regulator 1 (99.4%/15%) | 62.9 | No | 94% Ascomycota, 5% Basidiomycota, 1% others |
FGSG_04507 | 0.63 | C2 domain (1.1e-10, PF00168) | Endocytosis, exocytosis, synaptotagmin-1 (100%/54%) | 52.1 | No | 41% Metazoan, 29% Viridiplantae, 22% Ascomycota, 8% others |
FGSG_07909 | 0.62 | Homeobox KN domain (2.3e-15, PF05920) | Homeobox domain (99.7%/11%) | 84.6 | Yes | 56% Metazoan, 29% Viridiplantae, 12% Ascomycota, 3% others |
FGSG_07846 | 0.61 | FMO-like (5.8e-16, PF00734) | Monooxygenase (100%/75%) | 62.6 | No | 37% Bacteria, 32% Metazoan, 15% Viridiplantae, 12% Ascomycota, 4% others |
| Â | Pathogen | Â | Â | Â | Â | Â |
FGSG_04060 | 0.65 | Rare lipoprotein A like double-psi beta barrel (3.1e-05, PF03330) | Beta-expansin 1a (100%/94%) | 22.2 | Yes | 48% Bacteria, 16% Ascomycota, 13% Basidiomycota, 8% Phytophthora, 5% dictyostelium, 10% others |
FGSG_09841 | 0.65 | – | – | 20.8 | Yes | 76% Ascomycota, 15% Bacteria, 6% Archaea, 3% others |
FGSG_11496 | 0.64 | Rare lipoprotein A like double-psi beta barrel (3.1e-05, PF03330) | Beta-expansin 1a (100%/93%) | 25.2 | Yes | 23% Viridiplantae, 21% Bacteria, 18% Ascomycota, 13% Basidiomycota, 11% Phytophthora, 6% Dictyostelium, 8% others |
FGSG_03333 | 0.58 | Haloacid dehalogenase-like hydrolase (8e-10, PF12710) | Phosphorylcholine phosphatase (100%/90%) | 41.1 | Yes | 93% Bacteria, 7% Ascomycota |
FGSG_09148 | 0.55 | Peptidase C69 (3.1e-25, PF03577) | Acyl-coenzyme (99.2%/44%) | 57.2 | Yes | 90% Bacteria, 9% Eukaryota, 1% Archaea |
FGSG_04507 | 0.52 | C2 domain (1.1e-10, PF00168) | Endocytosis, exocytosis, synaptotagmin-1 (100%/54%) | 52.1 | No | 41% Metazoan, 29% Viridiplantae, 22% Ascomycota, 8% others |
FGSG_03549 | 0.52 | – | – | 28.2 | No | 100% Ascomycota |
FGSG_11152 | 0.49 | – | Coronatine-insensitive protein 1 (99.9%/94%) | 44.4 | No | 100% Ascomycota |
FGSG_09328 | 0.48 | Fungal specific transcription factor domain (3.5e-05, PF04082) | Centromere DNA-binding protein complex cbf3 (98.2%/72%) | 62.6 | No | 94% Ascomycota, 6% Basidiomycota |
FGSG_07909 | 0.48 | Homeobox KN domain (2.3e-15, PF05920) | Homeobox domain (99.7%/11%) | 84.6 | Yes | 56% Metazoan, 29% Viridiplantae, 12% Ascomycota, 3% others |