Figure 5

Growth characteristics and quasispecies distribution of vR26/P-4, vR26/P-12, vR26_E2gif/P-4 and vR26_E2gif/P-12. (A) Time courses of virus yields after infection of SFT-R cells with vR26/P-4, vR26/P-12, vR26_E2gif/P-4 and vR26_E2gif/P-12. Cells were infected with the viruses (MOI of 0.1 plaque-forming units per cell) and harvested after 3, 24, 48 and 72 h, as indicated, and the virus titres determined (n = 1). (B) Single nucleotide variant (SNV) data obtained from cDNA generated from the rescued viruses vR26/P-4, vR26/P-12, vR26_E2Gif/P-4 and vR26_E2Gif/P-12. The bar diagram depicts the quasispecies distribution (as % variant) in the data set from the 454 FLX by Lofreq SNV-caller above 2% in frequency. SNVs (nt position in the cDNA) leading to silent mutations (-) or non-silent mutations (e.g. D3431G) are illustrated.