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Table 1 Nucleotide and amino acid differences between the consensus cDNA sequences of the parental vaccine virus (vRiemser), the cloned BAC cDNA (pBeloR26) and cDNA of the rescued vR26/P-12

From: Efficient generation of recombinant RNA viruses using targeted recombination-mediated mutagenesis of bacterial artificial chromosomes containing full-length cDNA

Region

nt position

vRiemser (cDNA)

pBeloR26 (BAC cDNA)

vR26/P-12 (cDNA)

aa change (in pBeloR26)

Npro

695

G

A

A

E108K

Erns

1427

C

T

T

H352Y

E1

2364

T

C

C

I664T

E2

3068

G

A

A

D899N

NS2

4441

A

G

G

-

NS3

5500

A

G

G

-

5530

A

G

G

-

6043

T

C

C

-

6201

T

C

C

V1943A

NS5A

9589

T

C

C

-

NS5B

10079

A

G

G

M3236V

10134

A

G

G

K3254R

10272

A

G

G

K3300R

10665

G

A

G*

G3431D

3′ UTR

12128

T

A

A

 

12136

T

C

C

 

12137

C

T

T

 

12152

T

A

A

 
  1. * Nt position 10665 in vR26/P-12 is reverted from A to G as in the parental cDNA.
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BMC Genomics

ISSN: 1471-2164

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