Figure 3From: RNA-seq reveals the RNA binding proteins, Hfq and RsmA, play various roles in virulence, antibiotic production and genomic flux in Serratia sp. ATCC 39006Analysis of flagellin. A. Extracts of flagella were analyzed by SDS-PAGE. Two abundant protein bands were identified by mass spectrometry (MALDI-TOF) as flagellin (or3159, 41.4 kD) and a porin (or3073, 38.8 kD). B- D. Transmission electron micrographs of WT + vector (B), Δhfq + vector (C), and Δhfq + pHfq (D) are shown with the scale bar at the bottom of each image indicating a length of 1 μm. Samples were grown to early stationary phase the same as those used in the RNA-seq. The vector used was pQE80oriT and pHfq refers to pNB36. The strains analyzed were NMW9 (Δhfq), NMW7 (rsmA), NMW25 (rpoS), and NWX21 (carA, pigX, flhC).Back to article page