Analysis of flagellin. A. Extracts of flagella were analyzed by SDS-PAGE. Two abundant protein bands were identified by mass spectrometry (MALDI-TOF) as flagellin (or3159, 41.4 kD) and a porin (or3073, 38.8 kD). B- D. Transmission electron micrographs of WT + vector (B), Δhfq + vector (C), and Δhfq + pHfq (D) are shown with the scale bar at the bottom of each image indicating a length of 1 μm. Samples were grown to early stationary phase the same as those used in the RNA-seq. The vector used was pQE80oriT and pHfq refers to pNB36. The strains analyzed were NMW9 (Δhfq), NMW7 (rsmA), NMW25 (rpoS), and NWX21 (carA, pigX, flhC).