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Figure 7 | BMC Genomics

Figure 7

From: The chromatin modification by SUMO-2/3 but not SUMO-1 prevents the epigenetic activation of key immune-related genes during Kaposi’s sarcoma associated herpesvirus reactivation

Figure 7

Confirmation of data derived from ChIP-seq for IRF-1, IRF-2 and IRF-7 binding sites with SUMO-2/3 enrichment relevant to K-Rta induction of KSHV reactivation in BCBL-1 cells. Chromatin samples derived from K-Rta-inducible BCBL-1 cells before and after 12 hours of K-Rta induction were used in ChIP reactions with antibodies specific for SUMO-1 and SUMO-2/3. Following ChIP assay, the IRF binding sites within the promoters of the genes, which are indicated at the bottom of the figure, were amplified using qPCR. All reactions were run in triplicate and normalized against the input. Nonspecific IgG was used as the control ChIP antibody.

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BMC Genomics

ISSN: 1471-2164

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