Figure 1

H3K4me3 profiles at three different transcription start sites. Profiles in A and B show a typical bimoodal structure, while the peak displayed in C is more complex. Data from three different samples (WT, Resc, Cfp1-/-) and measured in two repeat experiments (AB.1: upper panel and AB.2: lower panel) are shown. Arrows indicate transcription starts sites and direction of transcription. Shown are normalised read counts. Note, that in contrast to coverage plots, reads are here only represented by their estimated mid points. The patterns for WT and Resc strongly resemble each other and while the signal in experiment AB.2 is noisier than in AB.1, the overall shapes are very similar. In the Cfp1-/- sample read coverage appears to be reduced in parts of the regions. However, the second example shows that a decrease in one part of the region can be compensated for by a gain of signal in an upstream region. All three examples were consistently called by MMDiff in both experiments, but not called by any other method.