Figure 1

Genome-wide distribution of Ss-LrpB binding regions. A. Ss-LrpB binding profile generated by ChIP-chip experiments across the entire S. solfataricus P2 genome. These experiments were performed using an Ss-LrpB specific nanobody® (three replicate experiments), and an irrelevant nanobody® (single mock ChIP-chip experiment), according to the colour code as indicated. S. solfataricus P2 genomic coordinates are mentioned on the horizontal axis. Underneath plotted profiles, genomic locations of Clustered Regularly Interspaced Short Palindromic Repeats (CRISPRs) (gold bars) and IS elements (red bars) are shown, as extracted from the UCSC archaeal genome browser [56]. Targets that exhibit Ss-LrpB binding in vitro, are indicated. B. Schematic overview of location categories in which ChIP-enriched regions can be classified with respect to genomic organization. ChIP-enriched regions are indicated by black horizontal bars, whereas ORFs are depicted by horizontal arrows. C. Classification of Ss-LrpB binding regions in location categories as mentioned in panel B. The central pie chart shows the portions of ChIP-enriched regions overlapping at least partially with an intergenic region (dark grey) and ChIP-enriched regions that are exclusively located in intragenic regions (light grey). Stacked column charts show further division into sub-categories of peaks overlapping intergenic and coding sequences, shown on the left and right side of the pie chart, respectively.