Figure 6

Overlap between Ss-LrpB and LysM binding profiles. A. Venn diagram illustrating shared binding regions between Ss-LrpB and LysM. B. Zoomed binding profiles of Ss-LrpB- and LysM-specific ChIP assays for three selected targeted regions, belonging to one of three classes: class I: binding region that contains an Ss-LrpB binding motif and exhibits binding to Ss-LrpB but not LysM in vitro; class II: binding region that contains a LysM binding motif and exhibits binding to LysM but not Ss-LrpB in vitro; class III: binding region that does not contain an Ss-LrpB or LysM binding motif and does not interact with any of the two proteins in vitro. Aligned with the binding profiles, the genomic organization is depicted by representing ORFs as arrows. C. EMSA demonstrating simultaneous binding of Ss-LrpB and LysM to a 348-bp probe encompassing the Ssot28 target. The positions of free DNA (F) and bound DNA (B) are indicated. The following protein concentrations have been used: 40 nM LysM, 320 nM Ss-LrpB, 500 nM Ss-LrpB-specific Nb and 500 nM E. coli PepA, which was used as a negative control.