Figure 7

Expression analysis of genes of which the coding regions are located close to a selection of Ss-LrpB binding regions. Tested genes are subdivided into the following classes: class I: gene associated to binding region bound by LysM and Ss-LrpB that contains an Ss-LrpB binding motif and exhibits binding to Ss-LrpB but not LysM in vitro; class II: gene associated to binding region bound by LysM and Ss-LrpB that contains a LysM binding motif and exhibits binding to LysM but not Ss-LrpB in vitro; class III: gene associated to binding region bound by LysM and Ss-LrpB that does not contain an Ss-LrpB or LysM binding motif and does not interact with any of the two proteins in vitro; class IV: gene associated to binding region solely bound by Ss-LrpB in vivo; class V: gene associated to region that is bound by Ss-LrpB in vitro but not in vivo. Relative gene expression ratios in an Ss-lrpB::lacS versus PBL2025 strain are normalized against tbp expression. Values are the average of biological quadruplicates and standard deviations represent the biological variation. An asterisk indicates a P-value of 0.085. All other tested genes render P-values > 0.1, indicating no significant differences in expression. Absence of Ss-lrpB expression in the Ss-lrpB::lacS strain has been confirmed (fold ratio = 0.0031 ± 0.0020; P-value = 0.0001).