Figure 4

Lay-out of AngHV1 ORFs showing kinetic class on basis of the R _i-PAA -analysis at t = 6 hpi. The gene layout of AngHV1 is based on [22] updated by van Beurden et al.[23]. Both strands are shown with the terminal repeats boxed. The locations of forward- and reverse-orientated ORFs are shown on the respective strands, labelled with the corresponding ORF number, and introns are depicted as thin lines connecting the exons. Identified polyA signals are depicted as small arrows on the respective strands, marked “A”. Kinetic class is indicated by colour, based on R_i-PAA-values at t = 6 hpi, corresponding to Additional file 2: Table S2. Red = immediate early gene, black = early gene, dark grey = early/late gene, light grey = late gene, white = ORFs potentially compromised by 3’-coterminality. Analysis was not performed for ORF57.5, ORF92A and ORF92B, and hence kinetic class could not be determined.