Brain regions of female B6. APBTgmice show amyloid plaques at 8–12 months of age. Brains of female B6.APBTg mice were assessed for plaque deposition from 8–12 months of age. Plaques were visualized using Thioflavin T (Thio T, blue). Plaques were very obvious in the cortex, hippocampus and other brain regions in all mice assessed. Size and number of plaques increased with increasing age. In general, plaques were associated with reactive astrocytes (as determined by glial fibrillary acidic protein (GFAP) staining, green). DAPI was used to identify nuclei. Scale bars; Upper panels = 100 μm, lower panels = 50 μm.