RNA sequencing data quality and verification of CYP2S1 depletion in human bronchial epithelial cells. A. Principal component analysis shows significant differences between biological samples with normal (SCRAM, red) and reduced (759, blue) expression of CYP2S1. B. Heat map analysis reveals similarities between the biological replicates within each condition. C The spots on the MA plot represent each gene within the analysis. The x-axis displays the log2 basal expression for each gene, while the y-axis represents the log2 change in expression relative to (SCRAM) control. FDR adjusted significant changes (p < 0.05) are represented as green dots. D. Comparison of CYP2S1 expression normalized to ACTB using either RNA-seq (grey) or qPCR (white). ** represents significant differences (p < 0.01) between CYP2S1 expression in SCRAM controls (no fill) and CYP2S1 depleted (vertical lines) cells.