Figure 4

Transcription patterns of P. sojae bZIP candidates. The heat map shows gene transcription patterns during 10 stages of the life cycle: mycelia (MY), zoosporangia (SP), zoospores (ZO), cysts (CY), germinating cysts (GC), and samples from 1.5, 3, 6, 12, and 24 h post-inoculation of soybean leaves (IF1.5 h to IF24 h). The color bar represents log₂ of gene transcripts per million reads (TPM) values, ranging from dark blue (0) to red (8.5). Black represents no expression. “**” following protein ID indicates that gene expression was not detected in all 10 libraries, and “*” denotes ambiguous data because of other identical sequences [31]. Transcript level data were collected into 11 clusters using HCL methods and MeV software, and marked in the column “cluster” (A to J). Column “H₂O₂ response” shows the patterns of transcriptional changes following H₂O₂ treatments at 30 min, 1 h, and 2 h, respectively. “U” and “D” refer to >1.2 fold elevation or -reduction respectively that are significant with a t-test P-value < 0.05, while “-“ means no significant change.