Figure 1
From: Morphogenesis-regulated localization of protein kinase A to genomic sites in Candida albicans
Epitope-tagged PKA in C. albicans. A. Immunoblotting to detect HA-tagged PKA subunits. Strains were grown in liquid YPD medium to OD600 = 0.6-0.8 and proteins in cell extracts (50 μg) were separated by SDS-PAGE (10% acrylamide), blotted onto PVDF membranes and probed using anti-HA antibody. Strains tested were CAF2-1 (WT: lanes 1, 5), AF1003 (TPK1HA: lane 2), AF1004 (TPK1HA: lane 3), AF1005 (TPK2HA: lane 4) and AF1007 (BCY1HA: lane 6). B. Fluorescence microscopy of transformant cells treated with rat anti-HA antibody, which was detected with FITC-labelled anti-rat antibody (green signal) and DAPI to detect nuclei (red signal); yellow overlay signals are indicated by white triangles. C. Filament formation of strains producing HA-tagged Tpk proteins. Strains CAF2-1 (wt), II (TPK1/tpk1), TPK7 (TPK2/tpk2), TPO7.4 (tpk2/tpk2), AF1003 (TPK1HA/TPK1), AF1004 (TPK1HA/tpk1) and AF1005 (TPK2HA/tpk2) were grown for 3 d/37°C on Spider agar and single colonies were photographed.