Effects of IL-4 and Stat6 on lysosomal gene expression as measured by quantitative PCR. RNA was obtained from triplicate cultures of wild-type or Stat6-deficient mouse bone marrow macrophages. For the panels in columns 1 and 2 macrophages were cultured for 5 days with 20 ng/ml M-CSF and then with 20 ng/ml IL-4 for an additional 6 or 24 hours as indicated. The data in column 3 were obtained from cultures grown ± M-CSF and IL-4 for 10 days as in Figure 5. RNA was reverse transcribed and analyzed by quantitative PCR as described in Methods. Oligonucleotides are listed in Additional file 13.