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Table 1 Yield of OST samples sequencing and quantification of tagged reads

From: A platform independent RNA-Seq protocol for the detection of transcriptome complexity

sample (sequencing technology)

n.passed filterreads

average read length (nt)

n.of reads with ≥ 1 tag

n. of reads after the tags elimination ≥ 40nt

n.of reads after the tags elimination ≥ 100nt

n.of reads after duplicates elimination ≥ 40nt

OST-78 (454)

504,879

312

65,514

538,312

446,877

-

OST-83 (454)

551,068

336

56,494

582,551

505,315

-

OST-78 [1] (MiSeq)

1,595,032

207

208,880

1,716,457

1,381,413

421,927

OST-78 [2] (MiSeq)

1,595,032

208

177,262

1,786,470

1,377,657

390,845

OST-83 [1] (MiSeq)

2,121,777

214

222,766

2,363,988

1,887,111

640,919

OST-83 [2] (MiSeq)

2,121,777

214

195,037

2,331,186

1,882,368

605,078

  1. Summary statistics on general throughput of the 454 and MiSeq sequencing of the OST-78 and OST-83 tumor sample show a similar percentage of tagged-reads per sample (11% on average). MiSeq reads were further subjected to the elimination of duplicated sequences. [1] and [2] indicate the forward and the reverse read dataset of the PE sequencing.