A platform independent RNA-Seq protocol for the detection of transcriptome complexity

BMC Genomics

Table 2 Comparison between the number of simulated tagged reads expected and observed

Sample	N.of reads with 1 Tag	N.of reads with 2 Tags	N.of reads with 3 Tags	N.of reads with 4 Tags	N.of reads with 5 Tag	N. of total tags
Expected	5	100	726	166	5	3,072
Observed	0	113	782	105	0	2,992

Simulated reads can contain at most 5 tags per sequence. The number of Tags found (observed) per read was computed from the tagspositions.txt file produced by Tag Find.

ISSN: 1471-2164